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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
15.63-1000 pg/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Serum, plasma and other biological fluids
- 灵敏度:
6 pg/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人P21激活酶1(PAK1)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human PAK1(p21 activated kinase1) Microsample ELISA Kit |
| 别名 | Alpha PAK; p21 activated kinase 1; p65 PAK; PAK 1; PAK1; PAKalpha |
| 货号 | ELK8847MS |
| 反应种属 | Human |
| Q13153 | Q13153 |
| 检测类型 | Sandwich |
| 灵敏度 | 6 pg/mL |
| 标准品 | 1000 pg/mL |
| 检测范围 | 15.63-1000 pg/mL |
| 样本类型 | Serum, plasma and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAK1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase;Metabolic pathway; |

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文献和实验相关实验
-89 个氨基酸的N末端调节区的部分p21-activated kinase (PAK-1)相结合。PAK-1的这个区域,被认为是p21偶联区域即P21binding domain (PBD),特异性的与活性形式的Rac1相作用,最终提供了一个优秀的工具以分离细胞样本中的活性Rac1. 细胞被镁盐缓冲溶液( magnesium lysis buffer)所裂解; 接着裂解液需要采用GST-agarose beads (not provided)进行预清除.在这一步,少量的细胞裂解液能够储存
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人P21激活酶1(PAK1)微量上样酶联免疫吸附检测试剂盒【Human PAK1(p21 activated kinase1) Microsample ELISA Kit】
¥1820 - 2600





