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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.136 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 人IgG-Fc片段低亲和力受体Ⅲb(FcgR3B)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human FcgR3B(Fc Fragment Of IgG Low Affinity IIIb Receptor) Microsample ELISA Kit |
| 别名 | CD16b; CD16-B; FCGR3-B; FCG3; FcgRIII; Low affinity immunoglobulin gamma Fc region receptor III-B |
| 货号 | ELK2798MS |
| 反应种属 | Human |
| O75015 | O75015 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.136 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FcgR3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FcgR3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FcgR3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FcgR3B in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | CD & Adhesion molecule; |

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文献和实验Production of Chimeric Heavy-Chain Antibodies
of camelid sdAb to human Fc. The cHCAb has a molecular size approximately half that of IgG (80 kDa vs. 150 kDa). Production is achieved through a transient expression with a human embryonic kidney (HEK) expression system, which can rapidly provide hundreds
fragment (Fab) region of the molecule. Part of the C region of the H chains forms the crystallizable fragment (Fc) region of the molecule. This region mediates effector mechanisms, such as complement activation, and is the portion of the immunoglobulin
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A, B and C neurotoxinI453Clostridium botulinum neurotoxin (BoNT) serotype A and BI, 78 mg/L[57]Clostridium botulinum neurotoxin, serotype AI, 450 mg/kg cells308Clostridium botulinum neurotoxin heavy chain fragment, serotype BI, 390 mg/g[58]Clostridium
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