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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.32-20 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
0.11 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 人基质金属蛋白酶3(MMP3)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human MMP3(Matrix Metalloproteinase 3) ELISA Kit |
| 别名 | SL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1 |
| 货号 | ELK10204 |
| 反应种属 | Human |
| P08254 | P08254 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.11 ng/mL |
| 标准品 | 20 ng/mL |
| 检测范围 | 0.32-20 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MMP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MMP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP3 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase;Tumor immunity;Infection immunity;Cardiovascular biology; |

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文献和实验相关实验
质表达无影响;E2对hOB MMP-2及TIMP-1 蛋白质表达无影响;E2对人成骨肉瘤MG-63细胞株和hOB MMP-2活性亦无影响。结论:雌激素不足可导致其对人成骨样细胞MMP-1的抑制作用减弱,促进骨吸收和骨基质分解,此可能为雌激素缺乏致骨质疏松的重要发病机制。四、雌二醇对人成骨肉瘤MG-63细胞株和成人成骨细胞膜型 基质金属蛋白酶-1的影响目的:膜型基质金属蛋白酶-1(membrane type 1-matrix metalloproteinase, MT1-MMP)是最近发现的一种可由
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人基质金属蛋白酶3(MMP3)酶联免疫吸附检测试剂盒【Human MMP3(Matrix Metalloproteinase 3) ELISA Kit】
¥1680 - 2400





