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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Guinea pig
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
0.058 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 豚鼠脑源性神经营养因子(BDNF)酶联免疫吸附检测试剂盒 |
| 英文名称 | Guinea pig BDNF(Brain Derived Neurotrophic Factor) ELISA Kit |
| 别名 | Neurotrophin; Abrineurin |
| 货号 | ELK8219 |
| 反应种属 | Guinea pig |
| O70183 | O70183 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.058 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig BDNF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig BDNF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig BDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig BDNF in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Cytokine;Infection immunity;Neuro science; |

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文献和实验Expression of Neurotrophic Factors such as GDNF in the E. coli System
Neurotrophic factors play an essential role in the growth, survival, and differentiation of neurons in the nervous system. Several well-characterized neurotrophic factors, such as nerve growth factor (NGF), brain-derived
Identification of Neurotrophic Factors by Molecular Biological Techniques
Many proteins have “neurotrophic” activities in in vitro or in vivo test systems. Originally these were discovered as certain neurotrophic activities with unknown molecular structures. Nerve growth factor (NGF), brain derived neurotrophic
Production of Recombinant Neurotrophic Factors
factor (NGF), and its related protein family members, brainderived neurotrophic factor (BDNF or NT-2), and neurotrophin3 (NT.-3). The potential of producing mg and larger quantities of these agents will expedite our understanding of their functions
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