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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
0.057 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 小鼠氯化物细胞内通道蛋白1(CLIC1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse CLIC1(Chloride Intracellular Channel Protein 1) ELISA Kit |
| 别名 | G6; NCC27; p64CLCP; hRNCC; Chloride channel ABP; Nuclear chloride ion channel 27; Regulatory nuclear chloride ion channel protein |
| 货号 | ELK7367 |
| 反应种属 | Mouse |
| Q9Z1Q5 | Q9Z1Q5 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.057 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CLIC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CLIC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CLIC1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CLIC1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Metabolic pathway; |

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文献和实验Measurement of Chloride Movement in Neuronal Preparations
Inglefield, J.R. and Schwartz‐Bloom, R.D. 1997. Confocal imaging of intracellular chloride in living brain slices: Measurement of GABAA receptor activity. J. Neurosci. Methods. 75:127‐135.
CFTR is the only member of the ABC (ATP-binding cassette) protein superfamily known to function as an ion channel. Most other ABC proteins are ATP-driven transporters, in which a cycle of ATP binding and hydrolysis, at intracellular
for degradation of their content. Carrier vesicles budding from early endosomes are delivered to different intracellular destinations such as the plasma membrane, the recycling endosomes or the trans -Golgi network. Thus, early endosomes undergo continuous
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