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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Mouse
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
0.064 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1960.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2800.0 |
| 中文名称 | 小鼠珠丝结构蛋白1(BFSP1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Mouse BFSP1(Beaded Filament Structural Protein 1) ELISA Kit |
| 别名 | CP115; CP94; LIFL-H; Filensin; Lens fiber cell beaded-filament structural protein CP 115; Lens intermediate filament-like heavy |
| 货号 | ELK7349 |
| 反应种属 | Mouse |
| A2AMT1 | A2AMT1 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.064 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BFSP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BFSP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BFSP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BFSP1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction;Ophthalmology & Otorhinolaryngology;Developmental science; |

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文献和实验哺乳动物细胞和悬浮哺乳动物细胞的培养 ◆疫苗生产中病毒颗粒的制备 ◆支持杂交瘤形成时的细胞融合 ◆支持单核细胞、巨噬细胞、上皮细胞和成纤维细胞系的细胞生长 适应的细胞类型 Cell lines and Cell types ● 235-1,MNQ Rat Pituitary 大鼠垂体脑细胞 ● B82 Mouse L cells –connective tissue 小鼠L细胞联结组织 ● COS1, COS
分子与DNA结合,再经PCR扩增,由此定量检测抗原使敏感性高于ELISA和RIA。 二、基本原理 : 免疫PCR主要由两个部分 组成,第一部分的免疫反应类似于普通的酶联免疫吸附试验(ELISA)的测定过程;第二部分即通常的PCR检测,抗原分子的量最终由PCR产物的多少来反映。第一步 中,首先用待测抗原(如牛血清白蛋白(ESA))包被微滴板孔,再加入相应的特异抗体,于是抗体就与固相上的抗原结合形成抗原抗体复合物,蛋白A-链亲合素(Protein A-Streptavidin)嵌合
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