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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.06 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人热反应蛋白12(HRSP12)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human HRSP12(Heat Responsive Protein 12) Microsample ELISA Kit |
| 别名 | PSP; P14.5; UK114; Ribonuclease UK114; 14.5 kDa translational inhibitor protein; Heat-responsive protein 12 |
| 货号 | ELK5235MS |
| 反应种属 | Human |
| P52758 | P52758 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.06 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HRSP12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HRSP12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HRSP12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HRSP12 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |

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文献和实验分子信标技术研究蛋白质所取得的结果。他们用分子信标来检测单链结合蛋白,实验发现,分子信标产生的荧光。所产生的荧光强度较分子信标与核酸结合时产生的荧光强度小,但其强度远远大于分子信标与核酸发生错配时产生的荧光强度。同时他们还发现分子信标不能与双链结合蛋白结合,产生荧光。 Nobuko Hamaguchi根据分子信标的原理设计了Aptamer信标,可以直接用来检测蛋白质。这一方法与传统的酶联免疫吸附法测定蛋白质相比,具有简单、灵敏等优点。但这一方法不能用于检测非特异性ssDNA结合蛋白,而且分子
【摘要】化学发光免疫测定方(Chemiluminescence immumo—assay,CLIA)重复性好、操作简便、快速、无放射性危害,临床很有价值。特异性阻断试验证明CLIA技术检测血清中抗原或抗体特异性强。敏感性试验结果表明CLIA方法检测血清中抗原或抗体的最低限度为0.05ng/ml,明显高于ELISA法和RIA法。【关键词】化学发光标记免疫测定;酶联免疫吸附技术;放射免疫测定技术[中图分类号]R446 [文献标识码]A [文章编号]1606—8025(2004)05-377
, lilie H, et al. Folding proteins. In:Creighton T E ed. Protein Function: A Practical Approach, 2nd.New York: IRL,1997,57-99 [7]CowleyDT, MackinRB. Expression, purification and charaterization of recombinant human proinsulin. FEBS Lett,1997,402:124
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