人真核翻译延伸因子1γ(EEF1g)酶联免疫吸附检测试剂盒【
Human EEF1g(Eukaryotic Translation Elongation Factor 1 Gamma) ELISA Kit】- ￥1820 - 2600
- ELK Biotechnology
- 31.25-2000 pg/mL
- 12.2 pg/mL
- 武汉
- ELK4204
- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
武汉益普生物科有限公司
- 检测范围：
31.25-2000 pg/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度：
12.2 pg/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人真核翻译延伸因子1γ(EEF1g)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human EEF1g(Eukaryotic Translation Elongation Factor 1 Gamma) ELISA Kit |
| 别名 | EF1G; GIG35 |
| 货号 | ELK4204 |
| 反应种属 | Human |
| P26641 | P26641 |
| 检测类型 | Sandwich |
| 灵敏度 | 12.2 pg/mL |
| 标准品 | 2000 pg/mL |
| 检测范围 | 31.25-2000 pg/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EEF1g. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EEF1g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EEF1g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EEF1g in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Developmental science; |
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文献和实验Genome‐Wide Annotation and Quantitation of Translation by Ribosome Profiling
. Schneider‐Poetsch, T., Ju, J., Eyler, D.E., Dang, Y., Bhat, S., Merrick, W.C., Green, R., Shen, B., and Liu, J.O. 2010. Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin. Nat
多核糖体mRNAs是酿酒酵母中mRNA无义介导降解途径的底物
摘要 In eukaryotic cells, premature termination of translation at nonsense codons has been implicated as the cause of a variety of posttranscriptional events, including rapid mRNA decay in the cytoplasm or the nucleus, altered splice site selection
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
, or unless they are unusually long.An illustration of the effect of annealing temperature on the specificity and on the yield of amplification of Human papillomavirus type 16 （HPV-16） is given below （Williamson and Rybicki, 1991: J Med Virol 33: 165-171）。Plasmid and biopsy
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