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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
1.57-100 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
0.62 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人再生胰岛衍生蛋白1β(REG1b)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human REG1b(Regenerating Islet Derived Protein 1 Beta) ELISA Kit |
| 别名 | PSPS2; REGH; REGI-BETA; REGL; Pancreatic Stone Protein; Pancreatic Thread Protein; Lithostathine 1 Beta; Secretory Pancreatic Stone Protein 2 |
| 货号 | ELK3474 |
| 反应种属 | Human |
| P48304 | P48304 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.62 ng/mL |
| 标准品 | 100 ng/mL |
| 检测范围 | 1.57-100 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human REG1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human REG1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human REG1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human REG1b in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Infection immunity; |

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文献和实验Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
our current knowledge regarding the isolation human bone marrow?derived progenitor cells for the paracrine stimulation of islet regeneration after transplantation into immunodeficient mouse models of diabetes. By using high aldehyde dehydrogenase (ALDHhi
of yeast genetics to delineate signaling events in vivo. Our laboratory has published two papers using this strategy to identify in vivo targets for protein phosphatases (9, 40). In one study (9), we identified physiological substrates for the Glc7p-Reg1p
Communications【4-5】和 Cells【6】发表的三篇文章,整理了人多能干细胞(hPSC)来源的胰岛样类器官培养方案。该培养过程遵循发育原理,由 hPSC 依次分化为定型内胚层 (DE)、胰腺祖细胞 (PP)、内分泌前体 (EP), 最终诱导发育为人胰岛样类器官(human Islet-like Organoid, hILO)。 图 1. hPSC 来源细胞诱导成为胰岛样类器官的培养方案示意图【7】 细胞来源 hPSC 培养试剂配方 包被液 hPSC培养基 Day1 Day
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