人碘甲腺原氨酸脱碘酶Ⅲ(DIO3)酶联免疫吸附检测试剂盒【H

uman DIO3(Iodothyronine Deiodinase III) ELISA Kit】

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规格：	48T	产品价格：	￥1960.0
规格：	96T	产品价格：	￥2800.0

中文名称	人碘甲腺原氨酸脱碘酶Ⅲ(DIO3)酶联免疫吸附检测试剂盒
英文名称	Human DIO3(Iodothyronine Deiodinase III) ELISA Kit
别名	ITDI3; TXDI3; Type-III 5'-deiodinase
货号	ELK0408
反应种属	Human
检测类型	Sandwich
灵敏度	0.11 ng/mL
标准品	20 ng/mL
检测范围	0.32-20 ng/mL
样本类型	Tissue homogenates and other biological fluids.
反应时间	3.5h
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DIO3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DIO3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DIO3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DIO3 in the samples is then determined by comparing the OD of the samples to the standard curve.
研究领域	Enzyme & Kinase;

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