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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
冷冻
- 保质期:
12个月
- 库存:
99
- 供应商:
上海圻明生物
- 规格:
μg
产品名称:IL17A活性蛋白,C-His,Mouse
规格:50μg/100μg
纯度:>90% as determined by SDS-PAGE.
用途:SDS-PAGE,WB,ELISA,Immunogen
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined
by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no
obvious degradation and precipitation were observed. The loss rate is less than 5% within the
expiration date under appropriate storage condition.
重组蛋白生产中应用广泛。蛋白标签是一种方便有效的工具,可以提高重组蛋白的溶解性和稳定性;便于目的蛋白的检测、示踪和纯化。
随着研究的需求和技术的发展,各种标签应运而生,多样化的标签可以满足不同的应用场景。许多重组蛋白在生产过程中溶解度较差,
可以使用融合标签以增加溶解度,融合标签可用于目的蛋白没有特异性抗体的多种应用,如Western Blot、免疫沉淀、ELISA等。
蛋白标签类型主要分为三类,适用于不同的应用场景:表位标签、亲和标签和荧光标签。
- 表位标签往往是短肽序列,可用于免疫学应用,如 Western Blot 和免疫共沉淀。最常用的表位标签有His、FLAG、HA等。
- 亲和标签一般较长,可增加蛋白溶解度,广泛应用于重组蛋白的纯化,如SUMO、Trx、MBP等。
- 荧光标签可用于活细胞和死细胞检测,最常用的荧光蛋白包括绿色荧光蛋白(GFP)、橙色荧光蛋白(OFP)、红色荧光蛋白(RFP)和黄色荧光蛋白(YFP)。它们被广泛用于影像学研究,如细胞定位和共表达实验。
注意:由于产品数量众多,具体详细产品说明书欢迎免费索取。
本公司产品仅供科研使用,不用于临床或其他诊断治疗用途。
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