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- Cayman
- 400062-12.5 ml
- 2026年04月15日
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文献和实验Clonality - X Chromosome Inactivation Assay
can be utilized. 1. Materials DNA sample (see Processing of Microdissected Tissue - DNA-based Analysis) Proteinase K (Sigma) Proteinase K buffer (0.05 M Tris-HCL, 0.001 M EDTA, 1% Tween 20, 0.1 mg/ml proteinase K, pH 8.0) Phenol:chloroform:isoamyl alcohol
Preparation of Microinjection Buffer
EDTA, pH 8.0 (autoclaved) 100 mM NaCl 1 ml of 5 M NaCl (autoclaved) 1x Polyamines 50 microliters 1000x Polyamines mix Autoclaved H2O up to 50 ml NOTE: Prepare fresh and discard unused microinjection buffer.
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
to get through these as quickly as possible. 1. Spin down beads @10K, 1min, RT. 2. Aspirate supernatant. 3. Wash with 1ml of the following buffers and mix by inverting 3 or 4 times. Only aspirate supernatant to 0.1ml marking so no beads are lost: o FA lysis buffer
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