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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
广州市左克生物
- CAS号:
1218816-71-7
- 规格:
10mM*1mL/1mg/5mg/10mg
| 规格: | 10mM*1mL | 产品价格: | ¥2607.8 |
|---|---|---|---|
| 规格: | 1mg | 产品价格: | ¥1190.0 |
| 规格: | 5mg | 产品价格: | ¥2975.0 |
| 规格: | 10mg | 产品价格: | ¥5100.0 |
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文献和实验1. Inoculate the entire colony in 5 ml YPD culture O.N. or 12 hours 2. Dilute into YPA medium (1% yeast extract, 2% Bacto-peptone, 2%KOAC) to OD600~ 0.2, Vigorous shaking. 3. For ts mutants, ~15hours at 23C, others ~10 hours at 30C
Coordinate Regulation of Sphingosine Kinase and Actin Dynamics
kinase 1 (SK1) exhibited constitutive- and stimulus-enhanced association with actin filaments and F-actin-enriched membrane fractions in both intact macrophages and an in vitro reconstitution assay, whereas SK1 bound G-actin only under stimulated
Isoform-Selective Assays for Sphingosine Kinase Activity
. Here, we describe methods for selectively detecting SK1 and SK2 activities in vitro, highlighting conditions that can discriminate between the activities of these two enzymes. The assays measure the production of 32 P-labelled S1P following the addition of exogenous
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