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文献和实验in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
. Wehave shown, using cell fractionation and Western blot analysis,that endogenous forms of APC are predominantly cytoplasmic,although a fraction of APC is localized to the nucleus, and thatAPC nuclear export activity is not abolished by truncating
Plasma Remove the cells from the samples by centrifugation at 2,000 x g for 10 minutes in a refrigerated centrifuge. Centrifugation at this force is necessary to deplete platelets from the sample. Transfer the supernatant to a clean, chilled
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