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文献和实验Expression and Purification Methods for the Production of Recombinant Human Complement Component C2
Human complement component C2 is a critical factor of the classical complement pathway. Here we provide a method for the production of recombinant human C2 (rhC2) protein for research purposes. The human complement component C2 (hC
Determining Alleles of the C2 Gene by Southern Blotting
The second component of human complement, C2, is a 102-kDa single-chain glycoprotein. It provides the catalytic subunit for the C3/C5 convertases of the classical and lectin pathways of complement activation. The single gene encoding C2 spans
1 in the human embryonic kidney (HEK293) cell line are introduced. In isolated cell culture mitochondria, the proton leak can be measured and modulators of UCP1 activity can be tested. As mouse UCP1 in this system shows native behaviour, this may be a suitable
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