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文献和实验Immortalised cultured cells are powerful tools to assess the function of ectopically expressed proteins. However, it must be ensured that the protein of interest is functional in the host system and display native behaviour. In particular
Expressing Full-Length Functional PfEMP1 Proteins in the HEK293 Expression System
weight, which further complicates expression of these proteins in heterologous systems. The high molecular weight Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) adhesins expressed on the surface of the infected erythrocytes
Generating stable cell lines in HEK293
Generating stable cell lines in HEK293 Prior to transfection, it is recommended that you linearize your pcDNA gene construct. Linearizing will decrease the likelihood of the vector integrating into the genome in a way
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