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文献和实验Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
of recombinant human IgGs after production and purification for further assays. Here, we describe a human IgG/Fc capture enzyme linked immunosorbent assay (ELISA) which is simple, robust, inexpensive and specific for all types of human IgGs and related Fc fusion
Automated Protein NMR Structure Determination in Solution
The main drawback of protein NMR spectroscopy today is still the extensive amount of time required for solving a single structure. The main bottleneck in this respect is the manual evaluation of the experimental spectra. A clear solution
Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells
cells HEK293T and suspension HEK293-6E cells. More than 10 �g/mL yield of scFv-Fc protein can be obtained every day for one to two weeks by transient production in HEK293T cells, whereas soluble HEK293-6E cells produce more than 100 �g/mL scFv-Fc protein
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