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文献和实验Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
of recombinant human IgGs after production and purification for further assays. Here, we describe a human IgG/Fc capture enzyme linked immunosorbent assay (ELISA) which is simple, robust, inexpensive and specific for all types of human IgGs and related Fc fusion
Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells
cells HEK293T and suspension HEK293-6E cells. More than 10 �g/mL yield of scFv-Fc protein can be obtained every day for one to two weeks by transient production in HEK293T cells, whereas soluble HEK293-6E cells produce more than 100 �g/mL scFv-Fc protein
1 in the human embryonic kidney (HEK293) cell line are introduced. In isolated cell culture mitochondria, the proton leak can be measured and modulators of UCP1 activity can be tested. As mouse UCP1 in this system shows native behaviour, this may be a suitable
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