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文献和实验Gel-elongation assay for type II fatty acid s
PhosphorImager scanner Procedure 1) FASII gel-elongation assay was done by preincubating 0.25 - 2 µg of E. coli or S. aureus lysate with a serial dilution of inhibitors at room temperature for 20 min in 50 µl
Gel-elongation assay for type II fatty acid synthesis
Procedure 1) FASII gel-elongation assay was done by preincubating 0.25 - 2 µg of E. coli or S. aureus lysate with a serial dilution of inhibitors at room temperature for 20 min in 50 µl of buffer containing 100 mM sodium
to protein bound to the PVDF membrane and scan image using PhosphorImager scanner Procedure 1) FASII gel-elongation assay was done by preincubating 0.25 - 2 µg of E. coli or S. aureus lysate with a serial dilution of inhibitors at room temperature for 20
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