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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
广州市左克生物
- 宿主:
Mouse
- 规格:
50ug/100ug/1mg
| 规格: | 50ug | 产品价格: | ¥1229.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥2035.0 |
| 规格: | 1mg | 产品价格: | 询价 |
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产品简介:
品牌:abinscience/艾必赛
货号:HY378437
产品名称:Anti-Human CD18/ITGB2 Antibody (60.3), APC
产品规格:50T/100T
反应种属:Human
应用实验:FCM, WB
靶点:Complement receptor C3 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunit beta, Integrin beta-2, MFI7, CD18, ITGB2
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文献和实验1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is used. 2. Aspirate media. Rinse cells with PBS, aspirate.3. Lyse cells in 200 uL of SDS sample buffer (100 ℃). Immediately scrape the cells off the plate with a rubber
% nonfat dry milk works well for anti-PCREB, but in my hands milk is not as good as BSA from USB (it seems to mask the epitope). Remove block solution and add primary antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000
ELISA Procedure for Measuring Serum Antibody Titer
of an antibody is shown in Figure 22. The titer was estimated to be 1 in 2400. Figure 22. Antibody titer graph (X = dilution corresponding to Ab titer) Further Methods for Evaluating Anti-peptide Antibodies If the antibodies are to be used as a reagent
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