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- 湖北省武汉市
- Grapevine chrome mosaic virus Viral genome-linked protein重组蛋白表达
- 2026年04月08日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：P13025
基因名：Viral genome-linked protein
蛋白名：Viral genome-linked protein
蛋白别名：
1A, 1B, 1C-VPg, 1D-PRO, 1E-POL, 3C-like protease, EC:2.7.7.48, EC:3.4.22.-, EC:3.6.4.-, Membrane-binding protein, NTB, NTP-binding protein, P1, P1A protein, Picornain 3C-like protease, Protease cofactor, Putative ATP-dependent helicase, RNA-directed RNA polymerase, RNA1 polyprotein, Viral genome-linked protein
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 | 基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
Viral genome-linked protein相关研究文献：
1. Nucleotide sequence of Hungarian grapevine chrome mosaic nepovirus RNA1. [2798128]
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文献和实验the virus-encoded replicase. In order to produce a cloned, infectious viral genome, the clone must be constructed in a binary vector and be longer than the full, unit-length viral genome. The clone can then be transferred by Agrobacterium -assisted
Agrobacterium -Mediated Transfer of Geminiviruses to Plant Tissues
of an Agrobacterium binary vector, but for RNA viruses with linear genomes a cDNA copy of the genome must be inserted between a promoter (usually, the cauliflower mosaic virus 35S promoter [CaMV 35S]) and a transcription termination signal (e.g., the nopaline synthase
Furovirus Isolation and RNA Extraction
. 1 ), which are distinguished by the nature of the protein(s) putatively involved in cell-to-cell movement. Subgroup 1 consists of SBWMV, which appears to have a movement protein (MP) of the type characterized by the 30-kDa protein of tobacco mosaic virus (TMV) (6 ). Subgroup
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