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- 湖北省武汉市
- Simian virus 40 Large T antigen重组蛋白表达
- 2026年04月08日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
大肠杆菌系统重组蛋白表达
- 提供商：
普健生物（武汉）科技有限公司
Uniprot号：P03070
基因名：Large T antigen
蛋白名：Large T antigen
蛋白别名：
DNA 3'-5' helicase large T antigen, EC:5.6.2.4, LT, LT-AG, Large T antigen
服务内容：
| 服务项目 | 客户提供 | 服务内容 | 服务周期 | 交付内容 |
|---|---|---|---|---|
| 大肠杆菌系统 蛋白表达 | 基因序列 | 方案沟通 | 1天 | 方案报告 |
| 密码子优化和基因合成 | 5天 | 基因合成报告 | ||
| 表达纯化测试 | 3天 | 表达纯化测试报告 | ||
| 1L表达及纯化 | 3天 | 蛋白样品(3-5mg)及报告 | ||
| 放大发酵及纯化 | 7天 | 纯化的蛋白及报告 |
案例展示：
Large T antigen相关研究文献：
1. The genome of simian virus 40. [205947]
2. Complete nucleotide sequence of SV40 DNA. [205802]
3. Independent expression of the transforming amino-terminal domain of SV40 large I antigen from an alternatively spliced third SV40 early mRNA. [8223482]
4. Phosphorylation of large tumour antigen by cdc2 stimulates SV40 DNA replication. [2552322]
5. T antigen is bound to a host protein in SV40-transformed cells. [218111]
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文献和实验2. Complete nucleotide sequence of SV40 DNA. [205802]
3. Independent expression of the transforming amino-terminal domain of SV40 large I antigen from an alternatively spliced third SV40 early mRNA. [8223482]
4. Phosphorylation of large tumour antigen by cdc2 stimulates SV40 DNA replication. [2552322]
5. T antigen is bound to a host protein in SV40-transformed cells. [218111]
, termed T-antigen (T-ag), all of the proteins required for DNA replication are supplied by cells that are permissive for SV40 replication (3 ). Because the virus depends on host proteins for replication (4 ), the SV40 system has been used to identify
Methods to Assess Inhibition of Adipocyte Differentiation by SV40 Large T Antigen
. Preadipocytes transformed by a variety of oncogenic proteins, such as the Simian virus 40 (SV40) large-tumor antigen (SVLT) or activated ras oncogene (1 –6 ) and others, fail to differentiate into adipocytes. However, the transformation and differentiation
Use of Simian Immunodeficiency Virus Vectors for Simian Embryonic Stem Cells
immunodeficiency virus-based lentivirus vector. When cynomolgus ES cells are transduced with a simian immunodeficiency virus vector encoding the green fluorescent protein (GFP) gene, a large fraction of cells (greater than 50%) fluoresce, and high levels of GFP
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