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文献和实验Revised amino-allyl labeling protocol
C indefinitely. D. Coupling Add 0.5 m L 1M sodium bicarbonate, pH 9.0 to 50 mM final. Check 1M stock solution periodically for fluctuations in pH. Monofunctional NHS-ester Cy3 (PA23001) and Cy5 dye (PA25001, Amersham) is supplied
Protocol for Reverse Transcription and Amino-allyl Coupling of RNA
in pH. Monofunctional NHS-ester Cy3 (PA23001) and Cy5 dye (PA25001, Amersham) is supplied as a dry pellet. Each tube is sufficient to label 10 reactions under normal conditions. Dissolve dry pellet in 20 mL DMSO. Aliquot 2 mL into 10 single
-BSA was prepared using an Alexa Fluor 488 protein labeling kit from Molecular Probes. Alexa488-BSA-Dig was prepared by labeling Alexa488-BSA with 3-amino-3-deoxydigoxigenin hemisuccinamide, succinimidyl ester (Molecular Probes). Cy3-BSA and Cy5-BSA
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