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- 2025年07月09日
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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
during apoptosis. The tests were performed on sections of rat, chichen, frog and fish thymus. All the thymus were fixed in Bouin's fixative and embedded in paraffin wax (3). A) Immunocytochemical procedure The low molecular weight DNA fragments
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
) Dissect embryos or small pieces of tissue (e.g. the size of a retina from an adult rat works well with this procedure) into PBS containing 2mM MgCl2 (PBS + Mg2+) on ice. PBS(10X) 80 g NaCl 2 g KCl 11.5g Na2HPO4 2 g KH2PO
secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
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