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1mg
| 产品名称 | Biotin-beta-Amyloid Peptide (1-42) |
| 产品编号 | TD-BP029747-2 |
| 产品描述 | beta-Amyloid (1-42). Implicated in Alzheimer's disease. |
| 分子量 | 4758.4 |
| 分子式 | C₂₁₃H₃₂₈N₅₈O₆₂S₂ |
| 序列 | Biotin-DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA |
| 存放说明 | Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. |
| 溶解度 | Solubility is batch-dependent. Please refer to the Protocol Booklet and the batch-specific CoA for more information. |
| 处理 | This product is supplied in one (or more) pack size which is freeze dried. Therefore the contents may not be readily visible, as they can coat the bottom or walls of the vial. Solvents listed may be unsuitable for use in biological experiments. These solvents are intended to enable solubilisation and mixing of components. We recommend that biologically unsuitable solvents are removed prior to solubilisation in experimental media. Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Amyloid β (1-42) human peptide should be initially dissolved according to this method: Add a small amount of 1% NH4OH directly to the lyophilized solid (50-100 μl should be sufficient for 1mg of peptide) Dilute to a concentration of 1mg/ml or less with your buffer. Vortex gently to mix (less than 1 minute). The peptide cannot be stored long term in 1% NH4OH, therefore it is important to immediately dilute the NH4OH/peptide solution with PBS or other buffer to a concentration of 1 mg/ml. |
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文献和实验in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained
Immunoscreening of gt11 Expression Libraries Using an Avidin-Biotin Detection System
binding of four molecules of biotin to one of avidin. This has an amplification ability that can be used to increase the sensitivity of detection of antibodies or other biotin-labeled molecules. Using antibodies raised to a purified peptide
Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
) Lyophilized biotinylated peptide (Synpep or American Peptide) DMSO 5 mM D‐biotin (see recipe
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