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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Esp3I (BsmBI) (10 U/µL)
- 规格:
200 units
描述
Esp3I (BsmBI)限制性内切酶能够识别CGTCTC(1/5)^位点,在Tango缓冲体系(+DTT)中于37°C达到最佳切割效果(同裂酶:BsmBI)。Thermo Scientific传统限制性内切酶产品包含了大量的高品质内切酶,这些酶经过优化,可在五种缓冲液体系中的其中一种中进行酶切,此外,还随酶提供通用Tango缓冲液,以便进行双酶切。所有内切酶在推荐的缓冲液和反应条件下都具有100%的活性。为保证内切酶的性能稳定,Thermo Scientific限制性内切酶反应缓冲液还预先混入了BSA,以增强酶的稳定性并结合可能存在于DNA制备产物中的污染物。特点:
• 优异的质量—严格的质量控制和业内领先的生产流程
• 使用颜色标记的五大便捷缓冲系统
• 包含适于双酶切的通用型Tango缓冲液
• 反应缓冲液中预先混入BSA
• 种类齐全,特异性限制性内切酶的广泛选择
应用
• 分子克隆
• 限制性酶切位点作图(Restriction site mapping)
• 基因分型
• Southern blotting
• 限制性片段长度多态性(RFLP)
规格
| Compatible Buffer: | 10x Buffer Tango |
|---|---|
| Enzyme: | Esp3I (BsmBI) |
| Methylation Sensitivity: | CpG methylation-sensitive, Not dam methylation-sensitive, Not dcm methylation-sensitive |
| Optimal Reaction Temperature: | 37° C |
| Product Size: | 200 units |
| Sensitive to Heat Inactivation: | Yes |
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文献和实验【共享】3i胶体金经典文献1-快速一步法免疫层析检测技术的发展
http://www.3i.com.cn/article/07.htm Development of Rapid One-Step Immunochromatographic Assay (快速一步法免疫层析检测技术的发展) *Graduate School of Biotechnology and †Department of Chemistry, Korea University, 5-ka, Anam-dong, Sungbuk-ku, Seoul 136-701
neuromelanin isolation and identification
washed in phosphate buffer, centrifuged at 10,000 G for 15 min at RT, and resuspended. This was repeated twice. The pellet was then resuspended in 0.1 N PBS for a final volume of 150 µl, frozen in liquid N2 and stored on dry ice. For purification
neuromelanin isolation and identification
in phosphate buffer, centrifuged at 10,000 G for 15 min at RT, and resuspended. This was repeated twice. The pellet was then resuspended in 0.1 N PBS for a final volume of 150 µl, frozen in liquid N2 and stored on dry ice. For purification of NM from cells
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