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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Mouse IgG
- 形态:
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
- 保存条件:
Store at -20°C as received.
- 克隆性:
Monoclonal
- 适应物种:
Mouse
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
TA398670
- 级别:
科研级
- 库存:
4周
- 供应商:
OriGene
- 宿主:
Goat
- 应用范围:
ELISA
- 抗体英文名:
Biotinylated Goat monoclonal to Mouse IgG
- 抗体名:
Biotinylated Goat monoclonal to Mouse IgG
- 规格:
50 ug
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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
Determination of class and subclass of monoclonal antibodies (MAbs) is important because several immunochemical and functional properties of antibodies depend on the isotype. Knowledge of the light-chain isotype (κ for 95% or λ
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
) Briefly, substitute your anti H+L pan-reactive secondary antibody with an anti-isotype heavy chain specific secondary. Be aware of possible odd crossreactivties (e.g. many goat anti mouse IgG 1 heavy chain do stain rat IgG 2a
Western Blotting with Monoclonal Ant
the enzyme conjugate anti-mouse IgG:HRPO (Catalog# M15345) 1:2000 in wash buffer containing 5% non-fat dry milk (or 1% BSA for phosphotyrosine antibodies). 3.Decant the wash buffer, add the diluted enzyme conjugate and incubate with agitation for 30 minutes
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