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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
Purified Mouse Anti-Rat CD45
- 规格:
1ml
Brand BD Pharmingen™ Alternative Name Ptprc; Lca; Leucocyte common antigen; RT7; T200 Concentration 250 µg/ml Isotype Mouse BALB/c IgG1, κ Reactivity Rat (QC Testing) Application Flow cytometry (Routinely Tested)
Immunohistochemistry-frozen, Immunohistochemistry-zinc-fixed (Tested During Development)
Immunohistochemistry-formalin (antigen retrieval required) (Not Recommended)
Immunogen Leukocyte Common Antigen-enriched Glycoprotein Fraction from Wistar Rat Thymocytes Entrez Gene ID 24699 Storage Buffer Aqueous buffered solution containing BSA, goat serum, and ≤0.09% sodium azide.
Description
The OX-1 antibody recognizes all molecular forms of CD45 (Leukocyte Common Antigen) on all hematopoietic cells except erythrocytes. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family; its intracellular (COOH-terminal) region contains two PTP catalytic domains while the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), and differing levels of glycosylation. The CD45 isoforms detected in rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.
Suggested Companion Products
| Cat No. | Description | Size | |
|---|---|---|---|
| 550337 | Biotin Goat Anti-Mouse Ig (Multiple Adsorption) Polyclonal RUO | 0.25 mg |
|
| 550946 | Streptavidin HRP RUO | 50 mL |
|
| 550880 | DAB Substrate Kit RUO | 500 Tests |
|
| 550878 | Purified Mouse IgG1 κ Isotype Control RUO | 1 mL |
|
| 550523 | IHC Zinc Fixative RUO | 1000 mL |
|
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文献和实验saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
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