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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Klrd1 rat monoclonal antibody, clone 15F.18D1, PE
- 抗体英文名:
Klrd1 rat monoclonal antibody, clone 15F.18D1, PE
- 靶点:
Klrd1
- 应用范围:
ELISA, FC, IF
- 宿主:
Rat
- 适应物种:
Mouse
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
AM31859RP-N
- 级别:
科研级
- 库存:
5周
- 供应商:
OriGene
- 克隆性:
Monoclonal
- 保存条件:
Store the antibody undiluted at 2-8°C.DO NOT FREEZE!
- 形态:
PBS containing 0.02% sodium azide (NaN3) as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.Label: PEState: Liquid purified Ig fractionLabel: R - Phycoerythrin
- 免疫原:
Murine T cell clone AE7 cellsDonor: Lewis Rat spleen cellsFusion Partner: Murine myeloma cell line SP2/0
- 规格:
50 ug
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
T-cell dependent antigen specific in situ staining (Pe)
6- Wash twice in TBS-T. 7- counterstain with rabbit anti B-Pe 1:2000 (0.5 µg/ml Cortex Biochem). You may add 1% mouse serum to the anti-Pe antibodies, unless you plan to use mouse monoclonal further (see below). 8- wash twice in TBS-T. 9- block
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