- ￥10388
- OriGene
- 美国
- CL007A
- 2026年04月01日
- CT, FC, IHC
- Rat
- Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Mouse Ly-2 thymocytes.Donor: (Lou x DA) F1 rat.Fusion Partner: myeloma Y3/Ag1.2.3.
- 形态：
PBS, no preservative, 0.2 µm filteredState: Azide FreeState: Liquid purified IgG fraction
- 保存条件：
Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.Avoid repeated freezing and thawing.
- 克隆性：
Monoclonal
- 适应物种：
Mouse
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
CL007A
- 级别：
科研级
- 库存：
5周
- 供应商：
OriGene
- 宿主：
Rat
- 应用范围：
CT, FC, IHC
- 靶点：
Cd8a
- 抗体英文名：
Cd8a rat monoclonal antibody, clone YTS169.4, Azide Free
- 抗体名：
Cd8a rat monoclonal antibody, clone YTS169.4, Azide Free
- 规格：
1 mg
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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Purification of Endotoxin Free mAbs
Cobbold 31/7/96 上一篇：Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas 下一篇：Hybridoma Production
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