- ￥5467
- OriGene
- 美国
- AM26032SU-N
- 2026年04月01日
- IHC, WB
- Mouse
- Canine, Chicken, Feline, Human, Mouse, Primate, Rabbit, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
SDS extract of Human myocardium.
- 形态：
State: SupernatantState: Liquid Tissue Culture Supernatant with 0.2% BSA and 15mM Sodium Azide.
- 保存条件：
Store the antibody undiluted at 2-8°C.
- 克隆性：
Monoclonal
- 适应物种：
Canine, Chicken, Feline, Human, Mouse, Primate, Rabbit, Rat
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
AM26032SU-N
- 级别：
科研级
- 库存：
4周
- 供应商：
OriGene
- 宿主：
Mouse
- 应用范围：
IHC, WB
- 抗体英文名：
pan Actin (pan alpha / gamma 2) mouse monoclonal antibody, clone HHF35, Supernatant
- 抗体名：
pan Actin (pan alpha / gamma 2) mouse monoclonal antibody, clone HHF35, Supernatant
- 规格：
1 ml
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文献和实验Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
Polyclonal and Monoclonal Antibodies
later again (2 + 2 week cycle). Monoclonal Antibody If titre is positive do one of the following 2 weeks later: I - Boost tail vein with 20 ug- 50 ug of Antigen in PBS and proceed with fusion on day 4. OR II - Boost
The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
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