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- 详细信息
- 文献和实验
- 技术资料
- 宿主:
不限
- 适应物种:
m,r,h
- 库存:
大量
- 抗原来源:
不限
- 供应商:
西宝生物
- 是否单克隆:
0
- 保存条件:
Store at 4° C,
- 规格:
200 µg/ml
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
.Take a 10-20μl aliquot and dilute with 400μl buffer.Read OD at 556 nm and calculate dye concentration based on the molar extinction coefficient of 50,000.D/P should be around 0.75 per tubulin monomer.
Fixation and Immunofluorescence of the Cytoskeleton (Mitchison Lab) Recycling Tubulin (Mitchison Lab) Labeling Tubulin and Quantifying Labeling Stoichiometry (Mitchison Lab) Tubulin
Microtubule (MT)/Organelle Motility Assays
Rapidly thaw and immediately place on ice one aliquot each of axonemes, Golgi or ER membranes, 45 uM tubulin, rat liver cytosol, and 20x energy regeneration system. Dilute the axonemes with PM Buffer to the proper dilution (determined
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