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文献和实验Negative Stain Electron Microscopy of Microtubules
paper into small slivers) Grids (200 mesh copper grids that have been formvar coated, carbon coated) Rinse (ddH2 O with 5 mM EGTA or as appropriate) II. Negative Staining Protocol 1. Glow discharge formvar and carbon coated
Negative Stain Electron Microscopy of Microtubules
for >1 year.) Filter strips (prepared by cutting Whatman #1 filter paper into small slivers) Grids (200 mesh copper grids that have been formvar coated, carbon coated) Rinse (ddH2 O with 5 mM EGTA or as appropriate) II
-fuchsine ** Phosphatases in decalcified, embedded tissue. ** Congo red for amyloid ** Cartilage staining with safranine ** Stain for Chlamydia (Castaneda's method). ** Which staining method for copper is best? ** Diastase (amylase) control
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