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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
peroxidase to the immobilized biotinylated antibodies, 6) wash away of free enzyme conjugates, and 7) quantification of immobilized antibody-enzyme conjugates by monitoring horseradish peroxidase activities in the presence of the substrate
DETECTION OF RECOMBINANT PROTEINS BY ELISA
2CO 3 slowly while stirring. Adjust pH to 8.6 and QS to 100 ml with H 2O. Substrate Solution (1 mg/ml paranitrophenyl phosphate PNPP) 100 mg capsule of PNPP (Sigma 104-100) 100 ml substrate buffer
should be titrated for optimal dilution) Add 100uL of second step antibody to wells and incubate for 1hr Repeat washing step Prepare substrate solution* Add 100uL of substrate to well and incubate at RT for 60min add stopping
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