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文献和实验Blue Native Gel Electrophoresis
Blue Native Gel Electrophoresis Stock solutions 49.5%T, 3%C Acrylamide 24 g acrylamide, 0.75 g bisacrylamide / 50 ml H2O Store at RT 3 x Gel buffer 150 mM BisTris-HCl, 1.5 M 6-amino-caproic acid, pH 7.0 Adjust pH to 7.0 with HCl at 4°C
Agarose Gel Electrophoresis for the Separation of DNA Fragments
. 6) Place the gel tray into the casting apparatus. Alternatively, one may also tape the open edges of a gel tray to create a mold. Place an appropriate comb into the gel mold to create the wells. 7) Pour the molten agarose
cell (Bio‐Rad) or SE 600 ruby dual cooled vertical unit (Hoefer) with clamps, glass plates, casting unit, and buffer chambers Thick spacers: 3‐mm spacers for the Bio‐Rad apparatus or for the Hoefer unit, conventional
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