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文献和实验Atomic Force Microscopy Assays for Evaluating Polyglutamine Aggregation in Solution and on Surfaces
) and synthetic polyQ peptides with atomic force microscopy (AFM) are described. Ex situ AFM can be used to characterize htt aggregate formation and morphology. In situ AFM allows for tracking the formation and fate of individual polyQ
Gold Nanoparticle Quantitation via Fluorescence in Solution and Cell Culture
This chapter provides a protocol for quantitative analysis of gold in solution as well as gold uptake by macrophages. A 96-well fluorescence assay was developed to be able to determine gold concentrations for a given gold nanoparticle
High-Flux X-Ray and Neutron Solution Scattering
Solution scattering is a diffraction technique that is used to study the overall structure of biological macromolecules in the solution state (1 –3 ). Although X-rays are diffracted by electrons and neutrons are diffracted by nuclei
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