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文献和实验Introduction Ethidium bromide (EtBr), 3,8-diamino-5-ethyl-6-phenylphenanthridinium bromide, is commonly used as a non-radioactive marker for identifying and visualizing nucleic acid bands in electrophoresis and other methods
E.Z.N.A. Cycle-Pure Kit Spin Protocol
步骤 1. Perform agarose gel/ethidium bromide electrophoresis to analyze PCR product. 2. Determine the volume of the PCR reaction, transfer the sample into a clean 1.5ml microcentrifuge tube, and add 4-5 volumes of Buffer CP. For PCR
of nonisotopic probes synthesized with Ambion's MAXIscript™ and BrightStar™ Psoralen-Biotin Kits. The alternative to UV shadowing is staining with ethidium bromide or acridine orange and requires subsequent extraction of the dye. The detection limit of UV
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