HIV-1 MN ENV-17

Virion Attachment and Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand-Binding Assay

The HIV-1 entry receptors are CD4 and a chemokine receptor (CCR5 or CXCR4). In addition it has recently been demonstrated that HIV-1 gp120 binds to and signals through integrin α4 β7 , the gut-homing receptor (Arthos et al., Nat Immunol

A Cell-Cell Fusion Assay to Monitor HIV-1 Env Interactions with Chemokine Receptors

for triggering the fusion-inducing conformational change in the Env protein have been only recently identified, permitting the study of HIV-1 Env-mediated membrane fusion in much greater detail (for review, see 1 ). These molecules, termed coreceptors

A Yeast Recombination-Based Cloning System to Produce Chimeric HIV-1 Viruses and Express HIV-1 Genes

technique to insert env sequences first into a yeast cloning vector and then into the common pNL4-3 virus backbone. This technique is not limited to the env gene, but can be used to build chimeric viruses with any HIV-1 gene or genetic element. This cloning