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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pEF1/His B（pEF1HisB）
- 库存：
大量
- 供应商：
联迈生物
- 规格：
1ug
pEF1/His B（pEF1HisB）
质粒名称: pEF1/His B
其他名称:
质粒大小（bp）: 6189
抗性: Ampicillin
质粒类型: Mammalian Expression
是否病毒: Nonviral
启动子: EF-1a
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: 6X His, Xpress
测序引物:
GenBank号:
其他属性: 5′ Sequencing 1 Primer:T7 Fwd
5′ Sequencing 1 Primer Sequence:5’d[TAATACGACTCACTATAGGG]3′
Selectable Marker:Neomycin
Notes:
质粒图谱:
质粒序列:
| 1 | AATATTATTG | AAGCATTTAT | CAGGGTTATT | GTCTCATGAG | CGGATACATA | 50 |
| 51 | TTTGAATGTA | TTTAGAAAAA | TAAACAAATA | GGGGTTCCGC | GCACATTTCC | 100 |
| 101 | CCGAAAAGTG | CCACCTGACG | TCGACGGATC | GGGAGATCTC | CCGATCCCCT | 150 |
| 151 | ATGGTGCACT | CTCAGTACAA | TCTGCTCTGA | TGCCGCATAG | TTAAGCCAGT | 200 |
| 201 | ATCTGCTCCC | TGCTTGTGTG | TTGGAGGTCG | CTGAGTAGTG | CGCGAGCAAA | 250 |
| 251 | ATTTAAGCTA | CAACAAGGCA | AGGCTTGACC | GACAATTGCA | TGAAGAATCT | 300 |
| 301 | GCTTAGGGTT | AGGCGTTTTG | CGCTGCTTCG | CGATGTACGG | GCCAGATATA | 350 |
| 351 | CGCGTTGACA | TTGATTATTG | ACTAGGCTTT | TGCAAAAAGC | TTTGCAAAGA | 400 |
| 401 | TGGATAAAGT | TTTAAACAGA | GAGGAATCTT | TGCAGCTAAT | GGACCTTCTA | 450 |
| 451 | GGTCTTGAAA | GGAGTGGGAA | TTGGCTCCGG | TGCCCGTCAG | TGGGCAGAGC | 500 |
| 501 | GCACATCGCC | CACAGTCCCC | GAGAAGTTGG | GGGGAGGGGT | CGGCAATTGA | 550 |
| 551 | ACCGGTGCCT | AGAGAAGGTG | GCGCGGGGTA | AACTGGGAAA | GTGATGTCGT | 600 |
| 601 | GTACTGGCTC | CGCCTTTTTC | CCGAGGGTGG | GGGAGAACCG | TATATAAGTG | 650 |
| 651 | CAGTAGTCGC | CGTGAACGTT | CTTTTTCGCA | ACGGGTTTGC | CGCCAGAACA | 700 |
| 701 | CAGGTAAGTG | CCGTGTGTGG | TTCCCGCGGG | CCTGGCCTCT | TTACGGGTTA | 750 |
| 751 | TGGCCCTTGC | GTGCCTTGAA | TTACTTCCAC | CTGGCTGCAG | TACGTGATTC | 800 |
| 801 | TTGATCCCGA | GCTTCGGGTT | GGAAGTGGGT | GGGAGAGTTC | GAGGCCTTGC | 850 |
| 851 | GCTTAAGGAG | CCCCTTCGCC | TCGTGCTTGA | GTTGAGGCCT | GGCCTGGGCG | 900 |
| 901 | CTGGGGCCGC | CGCGTGCGAA | TCTGGTGGCA | CCTTCGCGCC | TGTCTCGCTG | 950 |
| 951 | CTTTCGATAA | GTCTCTAGCC | ATTTAAAATT | TTTGATGACC | TGCTGCGACG | 1000 |
| 1001 | CTTTTTTTCT | GGCAAGATAG | TCTTGTAAAT | GCGGGCCAAG | ATCTGCACAC | 1050 |
| 1051 | TGGTATTTCG | GTTTTTGGGG | CCGCGGGCGG | CGACGGGGCC | CGTGCGTCCC | 1100 |
| 1101 | AGCGCACATG | TTCGGCGAGG | CGGGGCCTGC | GAGCGCGGCC | ACCGAGAATC | 1150 |
| 1151 | GGACGGGGGT | AGTCTCAAGC | TGGCCGGCCT | GCTCTGGTGC | CTGGCCTCGC | 1200 |
| 1201 | GCCGCCGTGT | ATCGCCCCGC | CCTGGGCGGC | AAGGCTGGCC | CGGTCGGCAC | 1250 |
| 1251 | CAGTTGCGTG | AGCGGAAAGA | TGGCCGCTTC | CCGGCCCTGC | TGCAGGGAGC | 1300 |
| 1301 | TCAAAATGGA | GGACGCGGCG | CTCGGGAGAG | CGGGCGGGTG | AGTCACCCAC | 1350 |
| 1351 | ACAAAGGAAA | AGGGCCTTTC | CGTCCTCAGC | CGTCGCTTCA | TGTGACTCCA | 1400 |
| 1401 | CGGAGTACCG | GGCGCCGTCC | AGGCACCTCG | ATTAGTTCTC | GAGCTTTTGG | 1450 |
| 1451 | AGTACGTCGT | CTTTAGGTTG | GGGGGAGGGG | TTTTATGCGA | TGGAGTTTCC | 1500 |
| 1501 | CCACACTGAG | TGGGTGGAGA | CTGAAGTTAG | GCCAGCTTGG | CACTTGATGT | 1550 |
| 1551 | AATTCTCCTT | GGAATTTGCC | CTTTTTGAGT | TTGGATCTTG | GTTCATTCTC | 1600 |
| 1601 | AAGCCTCAGA | CAGTGGTTCA | AAGTTTTTTT | CTTCCATTTC | AGGTGTCGTG | 1650 |
| 1651 | AGGAATTAGC | TTGGTACTAA | TACGACTCAC | TATAGGGAGA | CCCAAGCTGG | 1700 |
| 1701 | CTAGGTAAGC | TTACCATGGG | GGGTTCTCAT | CATCATCATC | ATCATGGTAT | 1750 |
| 1751 | GGCTAGCATG | ACTGGTGGAC | AGCAAATGGG | TCGGGATCTG | TACGACGATG | 1800 |
| 1801 | ACGATAAGGT | ACCTAAGGAT | CCAGTGTGGT | GGAATTCTGC | AGATATCCAG | 1850 |
| 1851 | CACAGTGGCG | GCCGCTCGAG | TCTAGAGGGC | CCGTTTAAAC | CCGCTGATCA | 1900 |
| 1901 | GCCTCGACTG | TGCCTTCTAG | TTGCCAGCCA | TCTGTTGTTT | GCCCCTCCCC | 1950 |
| 1951 | CGTGCCTTCC | TTGACCCTGG | AAGGTGCCAC | TCCCACTGTC | CTTTCCTAAT | 2000 |
| 2001 | AAAATGAGGA | AATTGCATCG | CATTGTCTGA | GTAGGTGTCA | TTCTATTCTG | 2050 |
| 2051 | GGGGGTGGGG | TGGGGCAGGA | CAGCAAGGGG | GAGGATTGGG | AAGACAATAG | 2100 |
| 2101 | CAGGCATGCT | GGGGATGCGG | TGGGCTCTAT | GGCTTCTGAG | GCGGAAAGAA | 2150 |
| 2151 | CCAGCTGGGG | CTCTAGGGGG | TATCCCCACG | CGCCCTGTAG | CGGCGCATTA | 2200 |
| 2201 | AGCGCGGCGG | GTGTGGTGGT | TACGCGCAGC | GTGACCGCTA | CACTTGCCAG | 2250 |
| 2251 | CGCCCTAGCG | CCCGCTCCTT | TCGCTTTCTT | CCCTTCCTTT | CTCGCCACGT | 2300 |
| 2301 | TCGCCGGCTT | TCCCCGTCAA | GCTCTAAATC | GGGGGCTCCC | TTTAGGGTTC | 2350 |
| 2351 | CGATTTAGTG | CTTTACGGCA | CCTCGACCCC | AAAAAACTTG | ATTAGGGTGA | 2400 |
| 2401 | TGGTTCACGT | AGTGGGCCAT | CGCCCTGATA | GACGGTTTTT | CGCCCTTTGA | 2450 |
| 2451 | CGTTGGAGTC | CACGTTCTTT | AATAGTGGAC | TCTTGTTCCA | AACTGGAACA | 2500 |
| 2501 | ACACTCAACC | CTATCTCGGT | CTATTCTTTT | GATTTATAAG | GGATTTTGCC | 2550 |
| 2551 | GATTTCGGCC | TATTGGTTAA | AAAATGAGCT | GATTTAACAA | AAATTTAACG | 2600 |
| 2601 | CGAATTAATT | CTGTGGAATG | TGTGTCAGTT | AGGGTGTGGA | AAGTCCCCAG | 2650 |
| 2651 | GCTCCCCAGC | AGGCAGAAGT | ATGCAAAGCA | TGCATCTCAA | TTAGTCAGCA | 2700 |
| 2701 | ACCAGGTGTG | GAAAGTCCCC | AGGCTCCCCA | GCAGGCAGAA | GTATGCAAAG | 2750 |
| 2751 | CATGCATCTC | AATTAGTCAG | CAACCATAGT | CCCGCCCCTA | ACTCCGCCCA | 2800 |
| 2801 | TCCCGCCCCT | AACTCCGCCC | AGTTCCGCCC | ATTCTCCGCC | CCATGGCTGA | 2850 |
| 2851 | CTAATTTTTT | TTATTTATGC | AGAGGCCGAG | GCCGCCTCTG | CCTCTGAGCT | 2900 |
| 2901 | ATTCCAGAAG | TAGTGAGGAG | GCTTTTTTGG | AGGCCTAGGC | TTTTGCAAAA | 2950 |
| 2951 | AGCTCCCGGG | AGCTTGTATA | TCCATTTTCG | GATCTGATCA | AGAGACAGGA | 3000 |
| 3001 | TGAGGATCGT | TTCGCATGAT | TGAACAAGAT | GGATTGCACG | CAGGTTCTCC | 3050 |
| 3051 | GGCCGCTTGG | GTGGAGAGGC | TATTCGGCTA | TGACTGGGCA | CAACAGACAA | 3100 |
| 3101 | TCGGCTGCTC | TGATGCCGCC | GTGTTCCGGC | TGTCAGCGCA | GGGGCGCCCG | 3150 |
| 3151 | GTTCTTTTTG | TCAAGACCGA | CCTGTCCGGT | GCCCTGAATG | AACTGCAGGA | 3200 |
| 3201 | CGAGGCAGCG | CGGCTATCGT | GGCTGGCCAC | GACGGGCGTT | CCTTGCGCAG | 3250 |
| 3251 | CTGTGCTCGA | CGTTGTCACT | GAAGCGGGAA | GGGACTGGCT | GCTATTGGGC | 3300 |
| 3301 | GAAGTGCCGG | GGCAGGATCT | CCTGTCATCT | CACCTTGCTC | CTGCCGAGAA | 3350 |
| 3351 | AGTATCCATC | ATGGCTGATG | CAATGCGGCG | GCTGCATACG | CTTGATCCGG | 3400 |
| 3401 | CTACCTGCCC | ATTCGACCAC | CAAGCGAAAC | ATCGCATCGA | GCGAGCACGT | 3450 |
| 3451 | ACTCGGATGG | AAGCCGGTCT | TGTCGATCAG | GATGATCTGG | ACGAAGAGCA | 3500 |
| 3501 | TCAGGGGCTC | GCGCCAGCCG | AACTGTTCGC | CAGGCTCAAG | GCGCGCATGC | 3550 |
| 3551 | CCGACGGCGA | GGATCTCGTC | GTGACCCATG | GCGATGCCTG | CTTGCCGAAT | 3600 |
| 3601 | ATCATGGTGG | AAAATGGCCG | CTTTTCTGGA | TTCATCGACT | GTGGCCGGCT | 3650 |
| 3651 | GGGTGTGGCG | GACCGCTATC | AGGACATAGC | GTTGGCTACC | CGTGATATTG | 3700 |
| 3701 | CTGAAGAGCT | TGGCGGCGAA | TGGGCTGACC | GCTTCCTCGT | GCTTTACGGT | 3750 |
| 3751 | ATCGCCGCTC | CCGATTCGCA | GCGCATCGCC | TTCTATCGCC | TTCTTGACGA | 3800 |
| 3801 | GTTCTTCTGA | GCGGGACTCT | GGGGTTCGAA | ATGACCGACC | AAGCGACGCC | 3850 |
| 3851 | CAACCTGCCA | TCACGAGATT | TCGATTCCAC | CGCCGCCTTC | TATGAAAGGT | 3900 |
| 3901 | TGGGCTTCGG | AATCGTTTTC | CGGGACGCCG | GCTGGATGAT | CCTCCAGCGC | 3950 |
| 3951 | GGGGATCTCA | TGCTGGAGTT | CTTCGCCCAC | CCCAACTTGT | TTATTGCAGC | 4000 |
| 4001 | TTATAATGGT | TACAAATAAA | GCAATAGCAT | CACAAATTTC | ACAAATAAAG | 4050 |
| 4051 | CATTTTTTTC | ACTGCATTCT | AGTTGTGGTT | TGTCCAAACT | CATCAATGTA | 4100 |
| 4101 | TCTTATCATG | TCTGTATACC | GTCGACCTCT | AGCTAGAGCT | TGGCGTAATC | 4150 |
| 4151 | ATGGTCATAG | CTGTTTCCTG | TGTGAAATTG | TTATCCGCTC | ACAATTCCAC | 4200 |
| 4201 | ACAACATACG | AGCCGGAAGC | ATAAAGTGTA | AAGCCTGGGG | TGCCTAATGA | 4250 |
| 4251 | GTGAGCTAAC | TCACATTAAT | TGCGTTGCGC | TCACTGCCCG | CTTTCCAGTC | 4300 |
| 4301 | GGGAAACCTG | TCGTGCCAGC | TGCATTAATG | AATCGGCCAA | CGCGCGGGGA | 4350 |
| 4351 | GAGGCGGTTT | GCGTATTGGG | CGCTCTTCCG | CTTCCTCGCT | CACTGACTCG | 4400 |
| 4401 | CTGCGCTCGG | TCGTTCGGCT | GCGGCGAGCG | GTATCAGCTC | ACTCAAAGGC | 4450 |
| 4451 | GGTAATACGG | TTATCCACAG | AATCAGGGGA | TAACGCAGGA | AAGAACATGT | 4500 |
| 4501 | GAGCAAAAGG | CCAGCAAAAG | GCCAGGAACC | GTAAAAAGGC | CGCGTTGCTG | 4550 |
| 4551 | GCGTTTTTCC | ATAGGCTCCG | CCCCCCTGAC | GAGCATCACA | AAAATCGACG | 4600 |
| 4601 | CTCAAGTCAG | AGGTGGCGAA | ACCCGACAGG | ACTATAAAGA | TACCAGGCGT | 4650 |
| 4651 | TTCCCCCTGG | AAGCTCCCTC | GTGCGCTCTC | CTGTTCCGAC | CCTGCCGCTT | 4700 |
| 4701 | ACCGGATACC | TGTCCGCCTT | TCTCCCTTCG | GGAAGCGTGG | CGCTTTCTCA | 4750 |
| 4751 | TAGCTCACGC | TGTAGGTATC | TCAGTTCGGT | GTAGGTCGTT | CGCTCCAAGC | 4800 |
| 4801 | TGGGCTGTGT | GCACGAACCC | CCCGTTCAGC | CCGACCGCTG | CGCCTTATCC | 4850 |
| 4851 | GGTAACTATC | GTCTTGAGTC | CAACCCGGTA | AGACACGACT | TATCGCCACT | 4900 |
| 4901 | GGCAGCAGCC | ACTGGTAACA | GGATTAGCAG | AGCGAGGTAT | GTAGGCGGTG | 4950 |
| 4951 | CTACAGAGTT | CTTGAAGTGG | TGGCCTAACT | ACGGCTACAC | TAGAAGAACA | 5000 |
| 5001 | GTATTTGGTA | TCTGCGCTCT | GCTGAAGCCA | GTTACCTTCG | GAAAAAGAGT | 5050 |
| 5051 | TGGTAGCTCT | TGATCCGGCA | AACAAACCAC | CGCTGGTAGC | GGTGGTTTTT | 5100 |
| 5101 | TTGTTTGCAA | GCAGCAGATT | ACGCGCAGAA | AAAAAGGATC | TCAAGAAGAT | 5150 |
| 5151 | CCTTTGATCT | TTTCTACGGG | GTCTGACGCT | CAGTGGAACG | AAAACTCACG | 5200 |
| 5201 | TTAAGGGATT | TTGGTCATGA | GATTATCAAA | AAGGATCTTC | ACCTAGATCC | 5250 |
| 5251 | TTTTAAATTA | AAAATGAAGT | TTTAAATCAA | TCTAAAGTAT | ATATGAGTAA | 5300 |
| 5301 | ACTTGGTCTG | ACAGTTACCA | ATGCTTAATC | AGTGAGGCAC | CTATCTCAGC | 5350 |
| 5351 | GATCTGTCTA | TTTCGTTCAT | CCATAGTTGC | CTGACTCCCC | GTCGTGTAGA | 5400 |
| 5401 | TAACTACGAT | ACGGGAGGGC | TTACCATCTG | GCCCCAGTGC | TGCAATGATA | 5450 |
| 5451 | CCGCGAGACC | CACGCTCACC | GGCTCCAGAT | TTATCAGCAA | TAAACCAGCC | 5500 |
| 5501 | AGCCGGAAGG | GCCGAGCGCA | GAAGTGGTCC | TGCAACTTTA | TCCGCCTCCA | 5550 |
| 5551 | TCCAGTCTAT | TAATTGTTGC | CGGGAAGCTA | GAGTAAGTAG | TTCGCCAGTT | 5600 |
| 5601 | AATAGTTTGC | GCAACGTTGT | TGCCATTGCT | ACAGGCATCG | TGGTGTCACG | 5650 |
| 5651 | CTCGTCGTTT | GGTATGGCTT | CATTCAGCTC | CGGTTCCCAA | CGATCAAGGC | 5700 |
| 5701 | GAGTTACATG | ATCCCCCATG | TTGTGCAAAA | AAGCGGTTAG | CTCCTTCGGT | 5750 |
| 5751 | CCTCCGATCG | TTGTCAGAAG | TAAGTTGGCC | GCAGTGTTAT | CACTCATGGT | 5800 |
| 5801 | TATGGCAGCA | CTGCATAATT | CTCTTACTGT | CATGCCATCC | GTAAGATGCT | 5850 |
| 5851 | TTTCTGTGAC | TGGTGAGTAC | TCAACCAAGT | CATTCTGAGA | ATAGTGTATG | 5900 |
| 5901 | CGGCGACCGA | GTTGCTCTTG | CCCGGCGTCA | ATACGGGATA | ATACCGCGCC | 5950 |
| 5951 | ACATAGCAGA | ACTTTAAAAG | TGCTCATCAT | TGGAAAACGT | TCTTCGGGGC | 6000 |
| 6001 | GAAAACTCTC | AAGGATCTTA | CCGCTGTTGA | GATCCAGTTC | GATGTAACCC | 6050 |
| 6051 | ACTCGTGCAC | CCAACTGATC | TTCAGCATCT | TTTACTTTCA | CCAGCGTTTC | 6100 |
| 6101 | TGGGTGAGCA | AAAACAGGAA | GGCAAAATGC | CGCAAAAAAG | GGAATAAGGG | 6150 |
| 6151 | CGACACGGAA | ATGTTGAATA | CTCATACTCT | TCCTTTTTC | 6189 |
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文献和实验相关实验
Phosphate‐buffered saline (PBS; Sigma, cat. no. D8537) pEF1αBirAV5‐neo plasmid (see Fig. ; available from the author upon request
2)、哺乳动物/真核表达载体 pcDNA3.1+ 不含任何标签的真核表达载体 amp/neo 5.4kb E. coli/mammals pcDNA3.1- 不含任何标签的真核表达载体 amp/neo 5.4kb E. coli/mammals pcDNA4/V5-His A, amp 含His标签的真核表达载体 amp/neo pcDNA 3.1(-) myc-hisA 含His标签的真核表达载体 amp/neo pcDNA 3.1(-) myc-hisB 含His标签
a look from the following website just by clicking "Tyrosine Phosphorylation and Dimerization" http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-43F85YV-2&_user=1111158&_coverDate=06%2F29%2F2001&_rdoc=1&_fmt=full&_orig=search
技术资料暂无技术资料 索取技术资料
pEF1/His B（pEF1HisB）
¥1 - 1880
