Phospho-MDM2 (Ser186/Ser188)
Polyclonal Antibody（多克隆抗体） | Phospho-MDM2 (Ser186/Ser188) Antibody | Phospho-MDM2 (Ser186/Ser188)抗体- ￥2454
- CUSABIO
- 武汉
- CSB-PA570037
- 2026年03月28日
- ELISA, IF
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Peptide sequence around phosphorylation site of Ser186/Ser188(H-K-S(p)-D-S(p)-I-S) derived from Human MDM2.
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
-20°C or -80°C
- 克隆性：
多克隆
- 标记物：
Non-conjugated
- 适应物种：
Human
- 抗原来源：
12个月
- 目录编号：
Homo sapiens (Human)
- 供应商：
武汉研升生物科技有限公司
- 宿主：
Rabbit
- 应用范围：
ELISA, IF
- 靶点：
MDM2
- 抗体英文名：
Phospho-MDM2 (Ser186/Ser188) Antibody
- 抗体名：
Phospho-MDM2 (Ser186/Ser188) Antibody
- 规格：
100μl
|
Product Name |
Phospho-MDM2 (Ser186/Ser188) Antibody |
|
Code |
CSB-PA570037 |
|
Aliases |
ACTFS antibody; Double minute 2 protein antibody; E3 ubiquitin-protein ligase Mdm2 antibody; Hdm 2 antibody; Hdm2 antibody; HDMX antibody; MDM 2 antibody; MDM2 antibody; MDM2 oncogene E3 ubiquitin protein ligase antibody; Mdm2 p53 E3 ubiquitin protein ligase homolog antibody; Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa antibody; MDM2_HUMAN antibody; MDM2BP antibody; Mouse Double Minute 2 antibody; MTBP antibody; Murine Double Minute Chromosome 2 antibody; Oncoprotein Mdm2 antibody; p53 Binding Protein Mdm2 antibody; p53-binding protein Mdm2 antibody; Ubiquitin protein ligase E3 Mdm2 antibody; Ubiquitin protein ligase E3 Mdm2 antibody |
|
Product Type |
Polyclonal Antibody |
|
Immunogen Species |
Homo sapiens (Human) |
|
Size |
100μl |
|
Uniprot ID |
Q00987 |
|
Immunogen |
Peptide sequence around phosphorylation site of Ser186/Ser188(H-K-S(p)-D-S(p)-I-S) derived from Human MDM2. |
|
Host Species |
Rabbit |
|
Species Reactivity |
Human |
|
Tested Applications |
ELISA, IF; IF:1:100-1:200 |
|
Clonality |
Polyclonal |
|
Isotype |
IgG |
|
Conjugate |
Non-conjugated |
|
Buffer |
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
|
Form |
Liquid |
|
Stroage |
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
|
Purification Method |
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi |
|
Target Names |
MDM2 |
|
Research Areas |
Epigenetics and Nuclear Signaling;Cancer;Cell biology |
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
dongreyuan 有的抗体名称上面写着Ab-33，比如，β-catenin（Ab-33）antibody，请问，Ab-33是什么意思？ bettyzyj 这个抗体是非磷酸化的抗体non-phospho-antibody ，33 代表的是基因位点 dongreyuan 谢谢了 yhwangcams bettyzyj wrote
TBS 和 5% NGS 中封闭样品。市售的含有酪蛋白的封闭溶液与磷酸化的一抗结合后易减弱信号；因此，我们建议不使用含有酪蛋白的封闭剂进行磷酸化特异性抗体的检测。以上针对封闭步骤所提建议均是在以 SignalStain. Boost IHC Detection Reagent 作为检测试剂的情况下提出的。如果选择使用其他检测试剂，我们则建议使用与二抗来源相同的血清进行封闭。抗体：Phospho–Histone H2A.X (Ser139) (20E3) Rabbit mAb #9718样本：石蜡
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