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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human NAT13
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
-20°C or -80°C
- 克隆性:
多克隆
- 标记物:
Non-conjugated
- 适应物种:
Human, Mouse, Rat
- 抗原来源:
12个月
- 目录编号:
Homo sapiens (Human)
- 供应商:
武汉研升生物科技有限公司
- 宿主:
Rabbit
- 应用范围:
ELISA, WB
- 靶点:
NAA50
- 抗体英文名:
NAA50 Antibody
- 抗体名:
NAA50 Antibody
- 规格:
100μl
|
Product Name |
NAA50 Antibody |
|
Code |
CSB-PA015468GA01HU |
|
Aliases |
ody; FLJ13194 antibody; hNAT5 antibody; hSAN antibody; Mak3 homolog (S. cerevisiae) antibody; Mak3 homolog antibody; N-acetyltransferase 13 (GCN5-related) antibody; N-acetyltransferase 13 antibody; N-acetyltransferase 5 antibody; N-acetyltransferase san homolog antibody; N-alpha-acetyltransferase 50 antibody; N-alpha-acetyltransferase 50; NatE catalytic subunit antibody; Naa50 antibody; NAA50_HUMAN antibody; NAT 5 antibody; NAT13 antibody; NAT5 antibody; NatE catalytic subunit antibody; San antibody |
|
Product Type |
Polyclonal Antibody |
|
Immunogen Species |
Homo sapiens (Human) |
|
Size |
100μl |
|
Uniprot ID |
Q9GZZ1 |
|
Immunogen |
Human NAT13 |
|
Host Species |
Rabbit |
|
Species Reactivity |
Human, Mouse, Rat |
|
Tested Applications |
ELISA, WB |
|
Clonality |
Polyclonal |
|
Isotype |
IgG |
|
Conjugate |
Non-conjugated |
|
Buffer |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles. |
|
Form |
Liquid |
|
Stroage |
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
|
Purification Method |
Antigen Affinity purified |
|
Target Names |
NAA50 |
|
Research Areas |
Cell biology |

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文献和实验DEAE-Sephadex A-50 提取法纯化多克隆抗体 DEAE-Sephadex A-50(简称A-50)为弱碱性阴离子交换剂,经过NaOH处理将Cl- 型转为OH- 型后,可吸附酸性蛋白。γ1球蛋白属中性蛋白,等电点为pH6.85~7.5,其余均属酸性蛋白。在溶液为pH8.0时,酸性蛋白均被A-50 吸附。从而可分离纯化IgG。 1. 批量吸附法 此法可在1天内完成提取过程,纯化前后样品体积变化不大,
the antibody, load the affigel concentrate onto the column and hold at room temperature for 10 minutes.(15) Wash with 50 ml 10 mM Tris 7.5 and then wash with 50 ml 10 mM Tris 7.5/500 mM NaCl. Elute with 5 ml 100 mM Glycine pH 2.5 into 1 ml 1M Tris 8.0.(16
mls of PBS. Put 200 ul (3 mg/ml) of the antibody to an eppendorf tube. Add 0.8 mls of PBS. Pellet 1 ml of the cell suspension and discard the PBS supernatant. Add the antibody solution to the cell pellet, gently resuspend the cells
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