- ￥7540
- Thermo
- A34807
- 2026年03月27日
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文献和实验Sphingomyelin Quantitation Post-choline Labeling of HL60 Cells
13) Dry down samples completely. 14) Resuspend dried lipids in 50 µl TMT buffer. 15) Vortex vigorously and sonicate via three 1 minute bursts, vortex and rest for 1 minute in between. 16) Pre-incubate tubes in 37°C water bath for 5 minutes
Sphingomyelin Quantitation Post-choline Labeling of HL6
the transferred samples in the -200C freezer. SMase assay 13) Dry down samples completely. 14) Resuspend dried lipids in 50 µl TMT buffer. 15) Vortex vigorously and sonicate via three 1 minute bursts, vortex and rest for 1 minute
Immunoprecipitation of metabolically labeled cells
- dilute with Super E to convenient volume; add protease inhibitors, cold Met and antibody. Some antibodies work only after the antigen has been denatured (e.g. Ab 877, Ab 471): to 50 ul of sample in TMT, add 5 µl 10% SDS and incubate at 100 o C
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