CELL SURFACE BIOTINYLATION

Analysis of CFTR Endocytosis by Cell Surface Biotinylation

Correct localization of cystic fibrosis transmembrane conductance regulator (CFTR) is critical to its function. Although an intracellular role for CFTR is still somewhat controversial (1 ), there is clear agreement on an important role for CFTR

Investigation of KATP Channel Endocytosis and Cell Surface Density by Biotinylation and Western Blotting

to be one of the key determinants of cell surface channel density and defects of this process have been linked to diseases relating to ion channel dysfunction. Biotinylation allows the selective labelling and isolation of surface exposed proteins

Proteomic Analysis of the Lymphocyte Plasma Membrane Using Cell Surface Biotinylation and Solution-Phase Isoelectric Focusing

. The hydrophobicity and large size of these proteins renders their analysis by conventional proteomic approaches using 2D-electrophoresis problematic, limiting our ability to evaluate alterations of cell surface architecture as a function of varying physiological