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- 2026年03月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
参照说明书
- 保质期：
参照说明书
- 英文名：
Mix and Go Competent Cells Strain BL21(DE3)
- 库存：
1
- 供应商：
北京博蕾德生物科技有限公司
- 规格：
1.0 ml
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文献和实验TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
Preparation of Competent Cells 制备感受态细胞
: not for use with DH5-Alpha cells).Time required :Day 1: OvernightDay 2: OvernightDay 3: 4 hours to grow culture2 hours to prepare the competent cellsProcedure:Day 1Streak out the E.coli strain on an LBM plate (no ampicillin!) to isolate colonies and incubate
60ml Glycerin To make: mix, pH down to 5.8 with diluted (about 0.2M) aectic acid, sterile filter, keep at 4 ℃. TfbII 500ml 10.47g Mops, dissolve in 300ml distilled water, adjust pH to pH7.0 with 10M NaOH
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