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contamination in real-time pcr negative control-Real-Tim
I am having a similar problem: We are running two negative controls for every GOI. We prepare mastermixes of the Fprimer, Rprimer and water...dispense into tubes. Then add app. template. We then mix the tubes and dispense into the PCR plate in triplicate
The In Situ PCR:Amplification and Detection in a Cellular Context
, substrate and primers, which must be optimized in addition to varying cofactors [Mg, primer-template ratios, etc.], one must consider other variables and potential problems, all of which have direct consequences in the success of this technique
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