HISTONE ACETYLTRANSFERASE 1 AN

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

to include a "no antibody" control sample. Adjust the final volume of each sample with lysis buffer if required. Sample volumes should be between 400-800µl/sample to allow ample mixing during the overnight incubation. Add 1µg of antibody to each sample

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications

a "no antibody" control sample. Adjust the final volume of each sample with lysis buffer if required. Sample volumes should be between 400-800µl/sample to allow ample mixing during the overnight incubation. Add 1µg of antibody to each sample and incubate

RNA-chromatin immunoprecipitations (RNA-ChIP) in mammalian cells

(including an equal amount of a normal IgG control) and immune complexes were allowed to form by slow mixing on a rotating platform at 4ºC overnight. Although antibody concentrations will vary, a guideline can be to begin within the range of 0.5�5µg/ml