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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -80°C.
- 保质期:
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
- 英文名:
Purified recombinant protein of Human chromosome 18 open reading frame 21 (C18orf21), transcript variant 1, full length, with N-terminal HIS tag, expressed in E. coli, 50ug
- 供应商:
OriGene
- 规格:
50 ug
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文献和实验Generation of Recombinant Adeno-Associated Virus
open reading frame (ORF) encodes four Rep proteins, Rep78, Rep68, Rep52, and Rep40. The right ORF encodes three Cap proteins, VP1, VP2, and VP3.
with 250 µl Pierce Coomassie protein assay reagent in 96-well ELISA dishes and reading at 650 nm on a plate reader).The foregoing affinity purification steps were carried out at 4ºC.The proteins were dialyzed against PBS and stored at –20ºC.Protein
Expression and Purification of Recombinant -Defensins and -Defensin Precursors in Escherichia coli
and expressed in E. coli BL21 RIS cells. Cells growing exponentially in nutrient-rich liquid medium are induced to express the recombinant protein by addition of 50 mM isopropyl β-d -1-thiogalactopyranoside for 3–6 h. After bacterial cells collected
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