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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -80°C.
- 保质期:
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
- 英文名:
Purified recombinant protein of Human chromosome 15 open reading frame 21 (C15orf21), transcript variant 3, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug
- 供应商:
OriGene
- 规格:
50 ug
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文献和实验Generation of Recombinant Adeno-Associated Virus
open reading frame (ORF) encodes four Rep proteins, Rep78, Rep68, Rep52, and Rep40. The right ORF encodes three Cap proteins, VP1, VP2, and VP3.
were passed through 1-ml nickel affinity columns (B-PER 6×His fusion protein purification kit from Pierce)that had been pre-equilibrated with 7 M GuHCl in TBS (0.15 M NaCl,50 mM Tris.HCl pH 7.5).The columns were washed thrice with 1 ml 7 M GuHCl
primers and ligated into a mammalian expression vector under the control of a CMV promoter. Identification of a plasmid possessing the open reading frame of the paraoxonase-1 gene was carried out by PCR analysis using gene specific primers. Confirmation
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