Purified recombinant protein o
f Mouse Wnt inhibitory factor 1 (cDNA clone MGC:7686 IMAGE:3497155), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug- ￥2900
- OriGene
- TP502510
- 2026年03月19日
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- 文献和实验
- 技术资料
- 保存条件：
Store at -80°C.
- 保质期：
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
- 英文名：
Purified recombinant protein of Mouse Wnt inhibitory factor 1 (cDNA clone MGC:7686 IMAGE:3497155), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
- 供应商：
OriGene
- 规格：
20 ug
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
Figure 27.4.1 Schematic diagram of overall protocol. Yeast clones containing cDNA constructs are plated on selection medium. Each clone is grown and protein expression is induced. Pellets of each clone are transferred into 96‐well boxes. Fusion
v468.Chapter 3 分泌型跨膜相关蛋白提纯及Wnt抑制活性
Purification and Wnt-Inhibitory Activities of SecretedFrizzled-Related Proteins Methods in Molecular Biology v468. chapter Abstract Recombinant expression of secreted Frizzled-related proteins (sFRPs) in mammalian expression systemsis
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
of the Wnt targetprotein b-catenin by immunoblot (see Sections 3.1 – 3.5 ); or 3. Partial purification of cellular GSK3 by cation exchange chromatography(12) , followed by assay (see Section 3.6 ). Thisapproach assumes that the inhibitory protein complex
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