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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -80°C.
- 保质期:
Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
- 英文名:
Purified recombinant protein of Mouse StAR-related lipid transfer (START) domain containing 5 (Stard5), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug
- 供应商:
OriGene
- 规格:
20 ug
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
Pin/pin head cleaning solution (Arrayit), optional 384‐well plate containing purified protein samples ( protocol
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
. Place the tube on a magnet for 1-2 minutes to separate the beads from the solution. 5) Transfer the supernatant containing the cells to a new tube. 3. Expansion of Mouse T Cells 1) Start with 1-1.5 × 106 purified T
Purification of Antiserum or Ascites by Protein A/G Chromatography
A and protein G bind about 20 mg of IgG per ml of conjugated agarose. Therefore, the binding capacity of a 10 ml (5 ml) column is 200 mg (100 mg) of IgG. A high-titer rabbit antiserum has roughly 5 mg/ml of IgG, mouse ascites has roughly 10 mg/ml of IgG
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